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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Soja. |
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Data corrente: |
23/02/2000 |
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Data da última atualização: |
23/02/2000 |
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Autoria: |
NEPOMUCENO, A. L. |
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Título: |
Physiological and molecular responses of plants to water deficit. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
[S.l.]: University of Arkansas, 1998. |
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Páginas: |
269p. |
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Idioma: |
Inglês |
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Notas: |
PhD. Thesis. |
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Conteúdo: |
Tolerance to drought in plants results from networks of mechanisms working isolated or in combination. Genotypes that differ in tolerance to water-deficit stress should have qualitative and/or quantitative differences in gene expression. Therefore, to identify and isolate genes, which may differ among four cotton genotypes with diverse responses to water deprivaton, Differential Display (DDRT-PCR) was used. These genotypes were first physiologically characterized in growth chamber and greenhouse experiments. Water and osmotic potential of roots and leaves, photosynthetic rate and relative water content were some of the physiological parameters used to measure plant responses during and after the water defficit applied. Cultivar Siokra L-23 and the wild type T-1521, two drought tolerant genotypes, showed a significant ability of osmotic adjust during water deficit. Photosynthetic rate and relative water content were maintained near control values in the two tolerant genotypes. However, cultivar CS 50 and Stoneville 506, showed significant sensitivity to the water deficit applied, confirming previous observations. These results guided subsequent work with DDRT-PCR using mRNA samples colected during these experiments. Fifty-two cDNA fragments differentially expressed during water deficit were identified using DD. The differentially displayed fragments were isolated from the polyacrylamide gels and cloned in pGEM-T vectors. After cloning they were sequenced using thermocycling dideoxynucleotide chain termination protocols. Search of gene banks revealed 17 clones with high homology to known genes, and 20 clones with low homology, while 15 clones had no homologous entries. Among the sequences that showed high homology was a heat shock protein that binds to calmodulin. This gene was expressed only in the water-deficit tolerant genotypes (Siokra L-23 and T-1521) during the stress. Another gene identified by homology was trehalose-6-phosphate synthase. This enzyme is part of the metabolic pathway for production of trehalose, a sugar known to osmoprotect cell membranes during desiccation. The differentially expressed gene sequences could have use in screening germplasm banks for similar genotypes, in plant physiology studies, and in molecular mapping of plant responses to water deficit. MenosTolerance to drought in plants results from networks of mechanisms working isolated or in combination. Genotypes that differ in tolerance to water-deficit stress should have qualitative and/or quantitative differences in gene expression. Therefore, to identify and isolate genes, which may differ among four cotton genotypes with diverse responses to water deprivaton, Differential Display (DDRT-PCR) was used. These genotypes were first physiologically characterized in growth chamber and greenhouse experiments. Water and osmotic potential of roots and leaves, photosynthetic rate and relative water content were some of the physiological parameters used to measure plant responses during and after the water defficit applied. Cultivar Siokra L-23 and the wild type T-1521, two drought tolerant genotypes, showed a significant ability of osmotic adjust during water deficit. Photosynthetic rate and relative water content were maintained near control values in the two tolerant genotypes. However, cultivar CS 50 and Stoneville 506, showed significant sensitivity to the water deficit applied, confirming previous observations. These results guided subsequent work with DDRT-PCR using mRNA samples colected during these experiments. Fifty-two cDNA fragments differentially expressed during water deficit were identified using DD. The differentially displayed fragments were isolated from the polyacrylamide gels and cloned in pGEM-T vectors. After cloning they were sequenced using thermocycling d... Mostrar Tudo |
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Palavras-Chave: |
Drought resistance; Genotypes; Plant pohysiology; Plant water relation; Plante response. |
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Thesagro: |
Água; Algodão; Deficiência Hídrica; Fisiologia; Genética Molecular; Genótipo; Gossypium Hirsutum; Planta; Relação Água-Planta; Resistência a Seca; Stress. |
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Thesaurus Nal: |
cotton; molecular genetics; water deprivation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03263nam a2200361 a 4500
001 1559664
005 2000-02-23
008 1998 bl uuuu m 00u1 u #d
100 1 $aNEPOMUCENO, A. L.
245 $aPhysiological and molecular responses of plants to water deficit.
260 $a[S.l.]: University of Arkansas$c1998
300 $a269p.
500 $aPhD. Thesis.
520 $aTolerance to drought in plants results from networks of mechanisms working isolated or in combination. Genotypes that differ in tolerance to water-deficit stress should have qualitative and/or quantitative differences in gene expression. Therefore, to identify and isolate genes, which may differ among four cotton genotypes with diverse responses to water deprivaton, Differential Display (DDRT-PCR) was used. These genotypes were first physiologically characterized in growth chamber and greenhouse experiments. Water and osmotic potential of roots and leaves, photosynthetic rate and relative water content were some of the physiological parameters used to measure plant responses during and after the water defficit applied. Cultivar Siokra L-23 and the wild type T-1521, two drought tolerant genotypes, showed a significant ability of osmotic adjust during water deficit. Photosynthetic rate and relative water content were maintained near control values in the two tolerant genotypes. However, cultivar CS 50 and Stoneville 506, showed significant sensitivity to the water deficit applied, confirming previous observations. These results guided subsequent work with DDRT-PCR using mRNA samples colected during these experiments. Fifty-two cDNA fragments differentially expressed during water deficit were identified using DD. The differentially displayed fragments were isolated from the polyacrylamide gels and cloned in pGEM-T vectors. After cloning they were sequenced using thermocycling dideoxynucleotide chain termination protocols. Search of gene banks revealed 17 clones with high homology to known genes, and 20 clones with low homology, while 15 clones had no homologous entries. Among the sequences that showed high homology was a heat shock protein that binds to calmodulin. This gene was expressed only in the water-deficit tolerant genotypes (Siokra L-23 and T-1521) during the stress. Another gene identified by homology was trehalose-6-phosphate synthase. This enzyme is part of the metabolic pathway for production of trehalose, a sugar known to osmoprotect cell membranes during desiccation. The differentially expressed gene sequences could have use in screening germplasm banks for similar genotypes, in plant physiology studies, and in molecular mapping of plant responses to water deficit.
650 $acotton
650 $amolecular genetics
650 $awater deprivation
650 $aÁgua
650 $aAlgodão
650 $aDeficiência Hídrica
650 $aFisiologia
650 $aGenética Molecular
650 $aGenótipo
650 $aGossypium Hirsutum
650 $aPlanta
650 $aRelação Água-Planta
650 $aResistência a Seca
650 $aStress
653 $aDrought resistance
653 $aGenotypes
653 $aPlant pohysiology
653 $aPlant water relation
653 $aPlante response
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Registro original: |
Embrapa Cerrados (CPAC) |
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| Registros recuperados : 623 | |
| 2. |  | NEPOMUCENO, A. L. Biossegurança envolvendo OGM em laboratórios: nova legislação. In: ENCONTRO NACIONAL SOBRE METODOLOGIAS DE LABORATÓRIO-MET, 11., 2006, Concórdia. Anais. Concórdia: Embrapa Suínos e Aves, 2006. p. 22. (Embrapa Suínos e Aves. Documentos, 112). Organizadores: Airton Kunz, Martha Mayumi Higarashi, Paulo Esteves, Cátia Silene Klein, Carlos Bernardi, Claudete Hara Klein, Anelise Sulzbach, Tânia Maria Biavatti Celant.| Biblioteca(s): Embrapa Soja. |
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| 5. | | NEPOMUCENO, A. L. A agricultura e as plantas geneticamente modificados. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Proceedings... Londrina: Embrapa Soybean, 2004. p. 1217-1220. Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| 12. | | PAIVA, E.; NEPOMUCENO, A. L. OGMs na agricultura brasileira e mundial. In: FALEIRO, F. G.; FARIAS NETO, A. L. de (Ed.). Savanas: desafios e estratégias para o equilíbrio entre sociedade, agronegócio e recursos naturais. Planaltina, DF: Embrapa Cerrados, 2008. cap. 24, p. 795-810.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Cerrados; Embrapa Soja. |
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| 14. | | NEUMAIER, N.; NEPOMUCENO, A. L. Manejo del agua. In: EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). El cultivo de la soja en los tropicos: mejoramiento y produccion. Roma: FAO, 1995. p.153-160. (Coleccion FAO: Produccion y Proteccion Vegetal, 27).| Biblioteca(s): Embrapa Soja. |
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| 15. | | NEUMAIER, N.; NEPOMUCENO, A. L. Water management. In: TROPICAL soybean: improvement and production. Rome: FAO, 1994. p. 153-160. (FAO Plant Production and Protection Series, 27). Prepared by EMBRAPA-National Soybean Research Center.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Soja. |
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| 18. | | NEPOMUCENO, A. L.; ABDELNOOR, R.; CARNEIRO, N. A biotecnologia sul-americana na agricultura. In: CONGRESO DE SOJA DEL MERCOSUR, 3., 2006, Rosário. Mercosoja 2006: conferencias plenarias, foros, workshops. Rosário: Associación de la Cadena de Soja Argentina, 2006. p. 31-33. Nome correto do segundo autor ABDELNOOR, R. V.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 623 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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